Performance of dihaploid wheat lines obtained via anther culture

Anther culture of iranian wheat germplasm using different media

Genetic Transformation of Wheat (triticum Aestivum L.) Anther Culture- Derived Embryos by Electroporation

This report describes the delivery of plasmid DNA into wheat anther culture derived embryos. Initial electroporation experiments were conducted using enzyme (NT-1) pretreatment, 500 F capacitance, and plasmid pAM2100, which carries the herbicide resistance gene, bar, and the screenable marker gene, Gus. Electroporated embryos were allowed to recover 10 days on regeneration medium and then trans...

Genetic Stability Developed for β-Carotene Synthesis in BR29 Rice Line Using Dihaploid Homozygosity

Obtaining transgenic crop lines with stable levels of carotenoids is highly desirable. We addressed this issue by employing the anther culture technique to develop dihaploid lines containing genes involved in β-carotene metabolism. First, we used Agrobacterium- mediated transformation to develop primary transgenic plants containing the β-carotene biosynthetic genes, phytoene synthase (psy) and ...

مقایسه روش‌های تلاقی گندم * ذرت و کشت بساک در تولید گیاهان هاپلویید گندم

The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...

New findings in strawberry (Fragaria × ananassa Duch.) anther culture

In this research, some independent experiments were carried out for investigating the effects of microspore developmental stage, bud pretreatments (cold, heat and Ca), and embryo induction medium on anther culture of some strawberry cultivars. The results showed that the percentages of responsive and embryogenic anthers in cultivar Paros in liquid medium were influenced not only by different de...